Modulation of muscarinic-receptor expression in human embryonic lung fibroblasts by platelet-derived growth factor

Platelet-derived growth factor (PDGF) is known to have regulatory control of a large number of cellular components, including various receptors. We show that muscarinic acetylcholine receptors of the m2 subtype on CCL 137 human fibroblasts in culture are affected by PDGF treatment. A time-dependent...

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Bibliographic Details
Published in:Biochemical journal 1990-09, Vol.270 (2), p.409-412
Main Authors: Koman, A, Durieu-Trautmann, O, Couraud, P O, Strosberg, A D, Weksler, B B
Format: Article
Language:English
Subjects:
Cell Line
Down-Regulation
Embryo, Mammalian
Fibroblasts - metabolism
Gene Expression Regulation - drug effects
Humans
Kinetics
Lung
Nucleic Acid Hybridization
platelet-derived growth factor
Platelet-Derived Growth Factor - pharmacology
Quinuclidinyl Benzilate - metabolism
Receptors, Muscarinic - genetics
RNA, Messenger - metabolism
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Summary:Platelet-derived growth factor (PDGF) is known to have regulatory control of a large number of cellular components, including various receptors. We show that muscarinic acetylcholine receptors of the m2 subtype on CCL 137 human fibroblasts in culture are affected by PDGF treatment. A time-dependent down-regulation is observed in steady-state RNA levels, followed by a decrease in ligand-binding capacity. Minimum RNA levels are attained at 11 h; minimum binding capacity is observed after 24 h of treatment. To our knowledge, this is the first example of negative gene control by PDGF.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2700409