Cholesterol increase in mitochondria : its effect on inner-membrane functions, submitochondrial localization and ultrastructural morphology

The effect of cholesterol incorporation on some functions of the mitochondrial inner membrane and on the morphology of rat liver mitochondria was studied. Basal ATPase and succinate dehydrogenase activities remained unchanged after cholesterol was incorporated into the mitochondria; however, uncoupl...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical journal 1993-02, Vol.289 (3), p.703-708
Main Authors: ECHEGOYEN, S, OLIVA, E. B, SEPULVEDA, J, DIAZ-ZAGOYA, J. C, ESPINOSA-GARCIA, M. T, PARDO, J. P, MARTINEZ, F
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - analysis
Adenosine Triphosphatases - drug effects
Adenosine Triphosphate - metabolism
Animals
Biological and medical sciences
Biological membranes
Cholesterol - metabolism
Cholesterol - pharmacology
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Intracellular Membranes - drug effects
Intracellular Membranes - enzymology
Membrane physicochemistry
Mitochondria - drug effects
Mitochondria - enzymology
Mitochondria - ultrastructure
Molecular biophysics
Rats
Serum Albumin, Bovine - pharmacology
Succinate Dehydrogenase - analysis
Succinate Dehydrogenase - drug effects
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of cholesterol incorporation on some functions of the mitochondrial inner membrane and on the morphology of rat liver mitochondria was studied. Basal ATPase and succinate dehydrogenase activities remained unchanged after cholesterol was incorporated into the mitochondria; however, uncoupled ATPase activity was partially inhibited. The presence of several substrates and inhibitors did not change the amount of cholesterol incorporated, which was localized mostly in the outer membrane. Electron-microscope observations revealed the presence of vesicles between the outer and inner membranes; these vesicles increased in number with the amount of cholesterol incorporated. The data suggest that cholesterol induces the formation of vesicles from the outer membrane, and modifies the activity of stimulated ATPase.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2890703