Phosphorylated dolichols in aging

The age-associated changes in the levels and synthesis of dolichyl phosphate and dolichyl diphosphate derivatives were investigated in brain and liver of 057B1/NNia mice. The total chloroform/methanol (2:1, v/v)-extractable phosphorylated dolichols of brain increased from 1.01 micrograms/g at 3 mont...

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Bibliographic Details
Published in:Biochemical journal 1990-02, Vol.265 (3), p.891-894
Main Authors: Pullarkat, R K, Pullarkat, P S, Morris, G N
Format: Article
Language:English
Subjects:
Aging - metabolism
Animals
Brain - metabolism
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Dolichol - metabolism
Dolichol Phosphates - metabolism
Glycoproteins - biosynthesis
Mice
Mice, Inbred C57BL
Microsomes - metabolism
Microsomes, Liver - metabolism
Phosphorylation
Spectrophotometry, Ultraviolet
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Summary:The age-associated changes in the levels and synthesis of dolichyl phosphate and dolichyl diphosphate derivatives were investigated in brain and liver of 057B1/NNia mice. The total chloroform/methanol (2:1, v/v)-extractable phosphorylated dolichols of brain increased from 1.01 micrograms/g at 3 months to 5.22 micrograms/g at 28 months of age. The long-chain dolichyl diphosphate oligosaccharide (Dol-PP-oligo) levels of brain increased from 0.82 microgram/g in 3 months to 2.8 micrograms/g in 28-month-old animals. However, in liver and in kidney, the levels of these components were unaffected by age. Incorporation of labelled glucose from UDP-glucose into dolichyl phosphate glucose and Dol-PP-oligo in brain microsomes was unaffected by age, whereas, in liver microsomes, the rates of synthesis of both components increased by 50-150%. The increased rate of synthesis and lack of accumulation of Dol-PP-oligo in liver suggest an active utilization and/or catabolism of these glycoprotein precursors. The accumulation of Dol-PP-oligo in aging brain may reflect its decreased utilization for N-glycosylation and/or reduced catabolism.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2650891