Bromelain Immobilized onto Clay-Carboxymethylcellulose Composites for Improving Nutritive Value of Soybean Meal

• Published in: ACS applied bio materials 2024-08, Vol.7 (8), p.5211-5221
• Main Authors: Pimcharoen, Kanlayanit, Opaprakasit, Pakorn, Yingchutrakul, Yodying, Simanon, Nattapon, Butkinaree, Chutikarn, Yuttayong, Darawan, Hompa, Ramawadee, Vayachuta, Lapporn, Prompinit, Panida
• Format: Article
• Language: English
• Subjects: Biocompatible Materials - chemistry; Bromelains - chemistry; Carboxymethylcellulose Sodium - chemistry; Clay - chemistry; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Glycine max - chemistry; Hydrolysis; Materials Testing; Nutritive Value; Particle Size
• ISSN: 2576-6422; 2576-6422
• DOI: 10.1021/acsabm.4c00392

Improvement of nutritional value and reduction of antinutritional factors (ANFs) of soybean meal (SBM) for animal feed applications could be achieved by using bromelain immobilized onto bentonite (Bt)-carboxymethylcellulose (CMC) composites. The composite with mass ratio between CMC to calcium ion (Ca2+) at 1:20 provided the highest enzyme activity, immobilization yield higher than 95%, with superior thermal and storage stabilities. Performance of the immobilized bromelain for soybean protein hydrolysis was further studied. The results showed that at 60 °C, the immobilized bromelain exhibited the highest efficiency in enzymatic hydrolysis to release free alpha amino nitrogen (FAN) as a product with high selectivity and to effectively reduce SBM allergenic proteins within 30 min. In conclusion, immobilization of bromelain onto Bt-CMC composites leads to stability enhancement of the enzyme, enabling effective improvement in SBM quality in a short treatment time and showing great potential for application in animal feed industries.