Differential influences of various arsenic compounds on glutathione redox status and antioxidative enzymes in porcine endothelial cells

The cellular response and detoxification mechanisms in porcine endothelial cells (PAECs) to arsenic trioxide (As2O3), sodium arsenite (NaAsO2) and sodium arsenate (Na2HAsO4) were investigated. NaAsO2 at 20 microM for 72 h increased Cu/Zn superoxide dismutase activity resulting in elevated intracellu...

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Published in:Cellular and molecular life sciences : CMLS 2002-11, Vol.59 (11), p.1972-1982
Main Authors: Yeh, J Y, Cheng, L C, Ou, B R, Whanger, D P, Chang, L W
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Antioxidants - metabolism
Aorta, Thoracic - drug effects
Aorta, Thoracic - metabolism
Arsenic
Arsenic - metabolism
Arsenicals - pharmacology
Blotting, Western
Cellular biology
Detoxification
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Enzymes
Glutathione - metabolism
Glutathione Disulfide - drug effects
Hogs
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Oxidation-Reduction - drug effects
Oxidative stress
Sodium arsenite
Sulfhydryl Reagents - pharmacology
Superoxide Dismutase - drug effects
Swine
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Summary:The cellular response and detoxification mechanisms in porcine endothelial cells (PAECs) to arsenic trioxide (As2O3), sodium arsenite (NaAsO2) and sodium arsenate (Na2HAsO4) were investigated. NaAsO2 at 20 microM for 72 h increased Cu/Zn superoxide dismutase activity resulting in elevated intracellular hydrogen peroxide levels, but As2O3 and Na2HAsO4 did not. Trivalent arsenic compounds increased intracellular oxidized glutathione (GSSG) and total glutathione (GSH) and cellular glutathione peroxidase (cGPX) and glutathione S-transferase (GST) activity, but not glutathione reductase activity. The increased cGPX activity resulted in an elevated cellular GSSG content. Na2HAsO4 increased the cellular GSSG level at 72 h compared to controls. These results imply that the increased GSH content responding to the oxidative stress by trivalent arsenic compounds may be mainly related to the regulation of GSH turnover. The increased GST activity implies that the elevated intracellular GSH level responding to the oxidative stress may be used to conjugate arsenic in PAECs and facilitate arsenic efflux.
ISSN:1420-682X
1420-9071
DOI:10.1007/PL00012519