Differential display analysis of gene expression in yeast

RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model f...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2002-08, Vol.59 (8), p.1241-1245
Main Authors: Ivanova, A V, Ivanov, S V
Format: Article
Language:English
Subjects:
Blotting, Northern
Cell Cycle Proteins - genetics
DNA Primers
Eukaryotes
Forecasting
Gene expression
Gene Expression Profiling
Histone-Lysine N-Methyltransferase
Methyltransferases
Polymerase Chain Reaction
Ribonucleic acid
RNA
Saccharomyces cerevisiae - genetics
Schizosaccharomyces - genetics
Schizosaccharomyces pombe Proteins
Yeast
Yeasts
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Description
Summary:RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome and simultaneously provides valuable information towards further refinement of this technique. Several examples discussed below illustrate how DD coupled with classical yeast genetic approaches may be used for studying transcriptionally regulated genetic systems.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-002-8502-y