Tamoxifen decreases the rate of proliferation of rat vascular smooth-muscle cells in culture by inducing production of transforming growth factor β

Tamoxifen selectively and reversibly decreased the rate of proliferation of adult rat aortic vascular smooth-muscle cells (VSMCs). Half-maximal inhibition of proliferation occurred at 2-5 microM tamoxifen for VSMCs and at > 50 microM for adventitial fibroblasts. The cell cycle time for all the VS...

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Bibliographic Details
Published in:Biochemical journal 1993-08, Vol.294 (1), p.109-112
Main Authors: GRAINGER, D. J, WEISSBERG, P. L, METCALFE, J. C
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Cell cultures. Hybridization. Fusion
Cell Cycle
Cell Division - drug effects
Cells, Cultured
DNA - biosynthesis
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Rats
Rats, Wistar
RNA, Messenger - metabolism
Tamoxifen - pharmacology
Transforming Growth Factor beta - biosynthesis
Transforming Growth Factor beta - genetics
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Summary:Tamoxifen selectively and reversibly decreased the rate of proliferation of adult rat aortic vascular smooth-muscle cells (VSMCs). Half-maximal inhibition of proliferation occurred at 2-5 microM tamoxifen for VSMCs and at > 50 microM for adventitial fibroblasts. The cell cycle time for all the VSMCs in the population was increased from 35 +/- 2 h to 54 +/- 4 h in the presence of 33 microM tamoxifen. Tamoxifen did not affect the time of entry into DNA synthesis, but delayed arrival at mitosis by > 24 h. It therefore extended the duration of the G2-to-M phase of the cell cycle. However, the rate of proliferation of VSMCs was not decreased by tamoxifen (at concentrations up to 50 microM) in the presence of neutralizing antibody to transforming growth factor beta (TGF-beta). The level of mRNA for TGF-beta 1 in VSMCs was strongly induced by 10 microM tamoxifen, and TGF-beta activity in conditioned medium from tamoxifen-treated cells was more than 50-fold higher than from control cells. Tamoxifen therefore extended the G2-to-M phase of the cell cycle in VSMCs by increasing TGF-beta activity in the culture.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2940109