Identification of a novel rat hepatic gene induced early by insulin, independently of glucose

We used mRNA differential display to identify new genes induced early after exposure to insulin. Our screening strategy was based on the comparison of gene expression during the time course of insulin induction in the liver of 12-day-old suckling rats both in vivo and in vitro. A novel, early induce...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical journal 2005-01, Vol.385 (Pt 1), p.165-171
Main Authors: Coffy, Sandrine, Decaux, Jean-François, Girard, Jean, de Keyzer, Yves, Asfari, Maryam
Format: Article
Language:English
Subjects:
Aging - genetics
Animals
Animals, Suckling
Biochemistry, Molecular Biology
Cells, Cultured
Diabetes Mellitus, Experimental - genetics
Estradiol - pharmacology
Fasting
Female
Gene Expression Profiling
Gene Expression Regulation - drug effects
Glucokinase - genetics
Glucose - pharmacology
Glucose Transporter Type 2
Hepatocytes - drug effects
Hepatocytes - metabolism
Insulin - pharmacology
Life Sciences
Liver - cytology
Liver - drug effects
Liver - embryology
Liver - metabolism
Male
Molecular biology
Molecular Sequence Data
Monosaccharide Transport Proteins - genetics
Proteins - genetics
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We used mRNA differential display to identify new genes induced early after exposure to insulin. Our screening strategy was based on the comparison of gene expression during the time course of insulin induction in the liver of 12-day-old suckling rats both in vivo and in vitro. A novel, early induced transcript, EIIH, was identified that encodes a 353-amino-acid protein with several features suggesting that it may be secreted or bound to membranes. EIIH is also distantly related to a variety of LRR (leucine-rich repeat) proteins. Insulin treatment increased EIIH mRNA levels in the hepatocytes of suckling, fasted adult and STZ (streptozotocin)-treated diabetic rats, where insulin was required to maintain the basal level of EIIH expression. EIIH expression was induced during the suckling/weaning transition, and remained detectable thereafter. Tissue distribution analysis in adult rats revealed a pattern of expression mainly in the liver, intestine and islets of Langerhans, closely following that of the Glut2 (glucose transporter 2), suggesting that it may play a role in carbohydrate metabolism. EIIH may be a primary target of the transcriptional regulation by insulin, and may therefore constitute a new model to study the mechanisms by which insulin acts on gene transcription.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj20040586