OsCYP706C2 diverts rice strigolactone biosynthesis to a noncanonical pathway branch

Strigolactones exhibit dual functionality as regulators of plant architecture and signaling molecules in the rhizosphere. The important model crop rice exudes a blend of different strigolactones from its roots. Here, we identify the inaugural noncanonical strigolactone, 4-oxo-methyl carlactonoate (4...

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Published in:Science advances 2024-08, Vol.10 (35), p.eadq3942
Main Authors: Li, Changsheng, Haider, Imran, Wang, Jian You, Quinodoz, Pierre, Suarez Duran, Hernando G, Méndez, Lucía Reyes, Horber, Robin, Fiorilli, Valentina, Votta, Cristina, Lanfranco, Luisa, Correia de Lemos, Samara M, Jouffroy, Lucile, Moegle, Baptiste, Miesch, Laurence, De Mesmaeker, Alain, Medema, Marnix H, Al-Babili, Salim, Dong, Lemeng, Bouwmeester, Harro J
Format: Article
Language:English
Subjects:
Agriculture
Biochemistry, Molecular Biology
Biomedicine and Life Sciences
Biosynthetic Pathways
Botanics
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Gene Expression Regulation, Plant
Genomics
Lactones - metabolism
Life Sciences
Mutation
Mycorrhizae - metabolism
Oryza - genetics
Oryza - metabolism
Phenotype
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
Plant Sciences
SciAdv r-articles
Vegetal Biology
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Summary:Strigolactones exhibit dual functionality as regulators of plant architecture and signaling molecules in the rhizosphere. The important model crop rice exudes a blend of different strigolactones from its roots. Here, we identify the inaugural noncanonical strigolactone, 4-oxo-methyl carlactonoate (4-oxo-MeCLA), in rice root exudate. Comprehensive, cross-species coexpression analysis allowed us to identify a cytochrome P450, OsCYP706C2, and two methyl transferases as candidate enzymes for this noncanonical rice strigolactone biosynthetic pathway. Heterologous expression in yeast and indeed demonstrated the role of these enzymes in the biosynthesis of 4-oxo-MeCLA, which, expectedly, is derived from carlactone as substrate. The mutants do not exhibit a tillering phenotype but do have delayed mycorrhizal colonization and altered root phenotype. This work sheds light onto the intricate complexity of strigolactone biosynthesis in rice and delineates its role in symbiosis and development.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.adq3942