Genome-Wide Identification and Characterization of Maize Long-Chain Acyl-CoA Synthetases and Their Expression Profiles in Different Tissues and in Response to Multiple Abiotic Stresses

• Published in: Genes 2024-07, Vol.15 (8), p.983
• Main Authors: Yan, Zhenwei, Hou, Jing, Leng, Bingying, Yao, Guoqi, Ma, Changle, Sun, Yue, Liu, Qiantong, Zhang, Fajun, Mu, Chunhua, Liu, Xia
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Adaptability; Analysis; Bioinformatics; Biosynthesis; Chloroplasts; Chromosomes, Plant - genetics; Coenzyme A Ligases - genetics; Coenzyme A Ligases - metabolism; Corn; Diseases and pests; Drought; Droughts; Endoplasmic reticulum; Environmental aspects; Enzymes; Fatty acid metabolism; Fatty acids; Gene duplication; Gene Expression Regulation, Plant; Gene regulation; Genes; Genetic aspects; Genome, Plant; Genome-wide association studies; Genomes; Growth; Health aspects; Lipid metabolism; Lipids; Metabolism; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Proteins; Regulatory sequences; Salt stress (Botany); Seeds; Stress, Physiological - genetics; Thioesters; Zea mays - genetics; Zea mays - metabolism
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes15080983

Long-chain acyl-CoA synthetases (LACSs) are essential enzymes that activate free fatty acids to fatty acyl-CoA thioesters, playing key roles in fatty acid (FA) catabolism, lipid synthesis and storage, epidermal wax synthesis, and stress tolerance. Despite their importance, comprehensive information about genes in maize, a primary food crop, remains scarce. In the present work, eleven maize genes were identified and mapped across five chromosomes. Three pairs of segmentally duplicated genes were detected in the maize gene family, which underwent significant purifying selection (Ka/Ks < 1). Subsequently, phylogenetic analysis indicated that genes were divided into four subclasses, as supported by highly conserved motifs and gene structures. On the basis of the PlantCARE database, analysis of the promoter regions revealed various cis-regulatory elements related to tissue-specific expression, hormonal regulation, and abiotic stress response. RT-qPCR analysis showed that genes exhibit tissue-specific expression patterns and respond to diverse abiotic stresses including drought and salt, as well as phytohormone abscisic acid. Furthermore, using the STRING database, several proteins involved in fatty acid and complex lipid synthesis were identified to be the potential interaction partners of ZmLACS proteins, which was also confirmed by the yeast two-hybrid (Y2H) assay, enhancing our understanding of wax biosynthesis and regulatory mechanisms in response to abiotic stresses in maize. These findings provide a comprehensive understanding of genes and offer a theoretical foundation for future research on the biological functions of genes in maize environmental adaptability.