Biosynthesis of Polyhydroalkanoates Doped with Silver Nanoparticles Using Pseudomonas putida and Pseudomonas aeruginosa for Antibacterial Polymer Applications

• Published in: International journal of molecular sciences 2024-08, Vol.25 (16), p.8996
• Main Authors: Cruz-Romero, Carmen Liliana, Chávez-Ramírez, Abraham Ulises, Flores-Juárez, Cyntia R, Arjona, Noé, Álvarez-López, Alejandra, Del Bosque Plata, Laura, Vallejo-Becerra, Vanessa, Galindo-de-la-Rosa, Juan de Dios
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacteria; Bacterial infections; Biodiesel fuels; Biofuels; Biopolymers; Biosynthesis; Carbon; Enzymes; Fatty acids; FDA approval; Glycerol; Glycerol - chemistry; Glycerol - metabolism; Kinases; Metabolism; Metabolites; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microorganisms; Nanoparticles; Oxidation; Polyhydroxyalkanoates - biosynthesis; Polyhydroxyalkanoates - chemistry; Polymers; Production costs; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - metabolism; Pseudomonas putida - metabolism; Reagents; Silver; Silver - chemistry; Silver - pharmacology
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25168996

In this study, the biosynthesis of polyhydroxyalkanoates (PHAs) was carried out using putida and . These PHAs were produced using reagent-grade glycerol and crude glycerol as the carbon sources. The objective was to compare the production of PHAs and to functionalize these polymers with silver nanoparticles to provide antibacterial properties for potential biomedical applications. The findings from the physical and chemical analyses confirmed the successful synthesis and extraction of PHAs, achieving comparable yields using both crude glycerol and reagent-grade glycerol as carbon sources across both strains. Approximately 16% higher PHAs production was obtained using compared to , and no significant difference was observed in the production rate of PHAs between the two carbon sources used, which means that crude glycerol could be utilized even though it has more impurities. Notably, PHAs functionalized with silver nanoparticles showed improved antibacterial effectiveness, especially those derived from reagent-grade glycerol and the strain.