Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms

Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phospho...

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Bibliographic Details
Published in:Biochemical journal 1995-08, Vol.310 (1), p.255-261
Main Authors: Bertello, L.E, Goncalvez, M.F, Colli, W, Lederkremer, R.M. de
Format: Article
Language:English
Subjects:
Animals
Bacillus thuringiensis - enzymology
Carbohydrate Sequence
Chromatography, Thin Layer
epimastigotes
Gas Chromatography-Mass Spectrometry
Molecular Sequence Data
Molecular Structure
myo-inositol
Phosphatidylinositol Diacylglycerol-Lyase
Phosphatidylinositols - chemistry
Phosphatidylinositols - isolation & purification
Phosphoinositide Phospholipase C
phospholipids
Phosphoric Diester Hydrolases - chemistry
plasma membrane
purification
structure
Trypanosoma cruzi
Trypanosoma cruzi - chemistry
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Summary:Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C16:0 and C18:0 fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrosphingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C18:2 and C18:1 fatty kids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3100255