Different molecular forms of rat kidney gp330, the dominant autoantigen of active Heymann nephritis

The primary structure, consisting of 1650 amino acid residues, of the C-terminal end of the dominant autoantigen of active Heymann Nephritis, gp330, from rat kidney was obtained by cloning and sequencing of cDNA clones. Comparison of this sequence with the previously published sequences of fragments...

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Bibliographic Details
Published in:Biochemical journal 1995-02, Vol.305 ( Pt 3) (3), p.711-713
Main Authors: Jokhadze, G G, Oleinikov, A V, Kanalas, J J, Makker, S P
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Autoantigens - chemistry
DNA, Complementary - chemistry
Glomerulonephritis - immunology
Heymann Nephritis Antigenic Complex
Kidney Glomerulus - immunology
Membrane Glycoproteins - chemistry
Molecular Sequence Data
Peptide Fragments - chemistry
Rats
Repetitive Sequences, Nucleic Acid
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Summary:The primary structure, consisting of 1650 amino acid residues, of the C-terminal end of the dominant autoantigen of active Heymann Nephritis, gp330, from rat kidney was obtained by cloning and sequencing of cDNA clones. Comparison of this sequence with the previously published sequences of fragments of the C-terminal end of gp330 [Raychowdhury, Niles, McCluskey and Smith (1989) Science 244, 1163-1165] revealed certain differences in their primary structures. These differences included several single amino acid substitutions, replacement of a stretch of 15 amino acid residues by a different stretch of six amino acid residues, and different lengths of cytoplasmic domain (188 versus 213 amino acid residues). These findings of two different primary structures of gp330 provide direct evidence for the existence of two molecular forms of gp330.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3050711