Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification

The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples, all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery afte...

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Bibliographic Details
Published in:Biochemical journal 1995, Vol.305 (1), p.337-343
Main Authors: Vuillard, L, Braun-Breton, C, Rabilloud, T
Format: Article
Language:English
Subjects:
analogs
Animals
betaine
Betaine - analogs & derivatives
Blood Proteins - isolation & purification
Detergents
Dialysis
Excipients
Isoelectric Focusing
Mice
Microsomes - chemistry
Nuclear Proteins - isolation & purification
Plasmodium falciparum
Protein Denaturation
protein extraction
Proteins - isolation & purification
Protozoan Proteins - isolation & purification
purification
Serine Endopeptidases - isolation & purification
Solubility
solubilization
Sulfonic Acids
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Description
Summary:The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples, all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery after dialysis of nuclear proteins; (3) reduction of precipitation in isoelectric focusing experiments under non-denaturing conditions; and (4) purification of a membrane-bound serine protease from Plasmodium falciparum involved in erythrocyte invasion by malaria merozoites. The absence of a significant denaturation effect induced by NDSBs is demonstrated by tests on beta-galactosidase and alkaline phosphatase. A simple NDSB synthesis and some possible explanations of the action of NDSBs are also presented.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3050337