Recruitment of mitochondrial cyclophilin to the mitochondrial inner membrane under conditions of oxidative stress that enhance the opening of a calcium-sensitive non-specific channel

Binding of mitochondrial matrix cyclophilin (CyP) to the rat liver mitochondrial membranes was detected by SDS/PAGE and Western blotting with suitable antipeptide antibodies. Binding was not affected by prior exposure of mitochondria to Ca2+, adenine nucleotides or inhibitors of the adenine nucleoti...

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Bibliographic Details
Published in:Biochemical journal 1994-09, Vol.302 (2), p.321-324
Main Authors: CONNERN, C. P, HALESTRAP, A. P
Format: Article
Language:English
Subjects:
Amino Acid Isomerases - metabolism
Amino Acid Sequence
Animals
Arsenicals - pharmacology
Biological and medical sciences
Blotting, Western
Calcium - metabolism
Carrier Proteins - metabolism
Cell structures and functions
Cyclosporine - pharmacology
Diamide - pharmacology
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Intracellular Membranes - drug effects
Intracellular Membranes - metabolism
Ion Channels - drug effects
Ion Channels - metabolism
Mitochondria and cell respiration
Mitochondria, Liver - drug effects
Mitochondria, Liver - metabolism
Molecular and cellular biology
Molecular Sequence Data
Peptidylprolyl Isomerase
Peroxides - pharmacology
Rats
tert-Butylhydroperoxide
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Summary:Binding of mitochondrial matrix cyclophilin (CyP) to the rat liver mitochondrial membranes was detected by SDS/PAGE and Western blotting with suitable antipeptide antibodies. Binding was not affected by prior exposure of mitochondria to Ca2+, adenine nucleotides or inhibitors of the adenine nucleotide translocase, but was greatly increased by t-butyl hydroperoxide (tBH), phenylarsine oxide or diamide. These all sensitized the opening of the non-specific mitochondrial pore to [Ca2+], and the effect of tBH was shown to be maintained after washing away the tBH, consistent with it being caused by the enhanced CyP binding. The bound CyP did not demonstrate peptidyl-prolyl cis-trans isomerase activity. CyP-binding was prevented by 5 microM cyclosporin A, but not reversed by cyclosporin treatment of the membranes. The effect of tBH on binding was concentration-dependent and maximal within 30 s.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3020321