Insulin stimulation of glucose transport activity in rat skeletal muscle : increase in cell surface GLUT4 as assessed by photolabelling

We have used a photoaffinity label to quantify cell surface GLUT4 glucose transporters in isolated rat soleus muscles. In this system, insulin stimulated an 8.6-fold increase in 3-O-methylglucose glucose transport, while photolabelled GLUT4 increased 8-fold. These results demonstrate that the insuli...

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Bibliographic Details
Published in:Biochemical journal 1994-05, Vol.299 (3), p.755-759
Main Authors: WILSON, C. M, CUSHMAN, S. W
Format: Article
Language:English
Subjects:
Affinity Labels
Animals
Azides
Biological and medical sciences
Biological Transport
Cell Membrane - metabolism
Disaccharides
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
Glucose Transporter Type 4
Glycosides
In Vitro Techniques
Insulin - pharmacology
Male
Monosaccharide Transport Proteins - chemistry
Monosaccharide Transport Proteins - metabolism
Muscle Proteins
Muscles - drug effects
Muscles - metabolism
Photochemistry
Propylamines
Rats
Rats, Sprague-Dawley
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
Citations: Items that cite this one
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Summary:We have used a photoaffinity label to quantify cell surface GLUT4 glucose transporters in isolated rat soleus muscles. In this system, insulin stimulated an 8.6-fold increase in 3-O-methylglucose glucose transport, while photolabelled GLUT4 increased 8-fold. These results demonstrate that the insulin-stimulated increase in glucose transport activity in skeletal muscle can be accounted for by an increase in surface-accessible GLUT4 content.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2990755