Evidence for a cell-specific negative regulatory element in the first intron of the gene for bovine elastin

A cell-specific negative regulatory element has been identified in the first intron of the gene for elastin in a region between 442 and 464 bp from the translational start site. This regulatory element functions both when it is located 5' of the promoter and 3' of the chloramphenicol acety...

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Bibliographic Details
Published in:Biochemical journal 1994-05, Vol.300 (1), p.147-152
Main Authors: MANOHAR, A, ANWAR, R. A
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Base Sequence
Biological and medical sciences
Cattle
Cells, Cultured
Chloramphenicol O-Acetyltransferase - genetics
Elastin - genetics
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Introns
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - metabolism
Oligodeoxyribonucleotides
Rats
Rats, Sprague-Dawley
Regulatory Sequences, Nucleic Acid
Transcription. Transcription factor. Splicing. Rna processing
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Summary:A cell-specific negative regulatory element has been identified in the first intron of the gene for elastin in a region between 442 and 464 bp from the translational start site. This regulatory element functions both when it is located 5' of the promoter and 3' of the chloramphenicol acetyltransferase (CAT) gene. The inhibition is observed both with the homologous elastin promoter and the heterologous SV1 promoter in transient expression experiments using rat aortic smooth-muscle cells. No inhibition was observed with NIH 3T3, Hep G2 and little, if any, with HeLa cells. Cell specificity was further confirmed by DNA mobility shift assays and the position of the negative regulatory element was localized with the use of synthetic duplex oligomers. It is proposed that this negative element plays a significant role in the modulation of the expression of the gene for elastin in the smooth-muscle cells of the aorta during development.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3000147