Microvillus inclusion disease: specific diagnostic features shown by alkaline phosphatase histochemistry

A technique using alkaline phosphatase histochemistry on routine sections of four jejunal biopsy specimens and one necropsy sample was applied to show that alkaline phosphatase activity, normally present in the brush border, occurs in the enterocytes of patients with microvillus inclusion disease. S...

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Bibliographic Details
Published in:Journal of clinical pathology 1988-08, Vol.41 (8), p.880-882
Main Author: Lake, B D
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - analysis
Atrophy
Biological and medical sciences
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Intestinal Diseases - congenital
Intestinal Diseases - diagnosis
Intestinal Diseases - enzymology
Jejunum - enzymology
Malformations
Medical sciences
Microvilli - enzymology
Microvilli - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
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Summary:A technique using alkaline phosphatase histochemistry on routine sections of four jejunal biopsy specimens and one necropsy sample was applied to show that alkaline phosphatase activity, normally present in the brush border, occurs in the enterocytes of patients with microvillus inclusion disease. Sections were cut at 5 micron, mounted on to glass slides, and dried overnight at 37 degrees C before staining for alkaline phosphatase activity by the indoxyl phosphatase nitro blue tetrazolium method. Incubation periods amounted to 10 minutes for biopsy specimens and 30 minutes to one hour for necropsy samples. The demonstration of alkaline phosphatase activity in routinely processed biopsy specimens provides an effective, quick, and definitive test in the diagnosis of microvillus inclusion disease without recourse to electron microscopy.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.41.8.880