Comparison of Y1 mouse adrenal cell and coagglutination assays for detection of Escherichia coli heat labile enterotoxin

A commercial coagglutination assay (COA; Phadebact LT-ETEC) was compared with a Y1 mouse adrenal cell assay for detecting the heat labile enterotoxin of Escherichia coli. Of four different media evaluated for use with the COA, only one (modified blood agar) gave a positive result with all strains kn...

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Bibliographic Details
Published in:Journal of clinical pathology 1989-07, Vol.42 (7), p.755-758
Main Authors: Chapman, P A, Daly, C M
Format: Article
Language:English
Subjects:
Adrenal Glands - cytology
Agglutination Tests
Animals
Bacterial Toxins - analysis
Bacteriology
Biological and medical sciences
Biological Assay
Cells, Cultured
Enterotoxins - analysis
Escherichia coli
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Mice
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
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Summary:A commercial coagglutination assay (COA; Phadebact LT-ETEC) was compared with a Y1 mouse adrenal cell assay for detecting the heat labile enterotoxin of Escherichia coli. Of four different media evaluated for use with the COA, only one (modified blood agar) gave a positive result with all strains known to produce heat labile enterotoxin. With modified blood agar, the COA detected 74 (85%) of 87 such strains. Eighty six strains negative by cell culture assay were also negative by COA, and one strain positive by COA could not be confirmed by cell culture. The Phadebact LT-ETEC kit provides a simple, sensitive, and economical method for detecting E coli heat labile enterotoxin.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.42.7.755