Susceptibility of Mycobacterium avium sbsp paratuberculosis to monensin sodium or tilmicosin phosphate in vitro and resulting infectivity in a murine model

This study was designed to determine the susceptibility in vitro and infectivity of 1 field isolate of Mycobacterium avium sbsp paratuberculosis after exposure to monensin sodium and tilmicosin phosphate. Minimum inhibitory concentrations (0.39 microg monensin sodium/mL; 1.60 microg tilmicosin phosp...

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Bibliographic Details
Published in:Canadian journal of veterinary research 2004-07, Vol.68 (3), p.175-181
Main Authors: Brumbaugh, G.W, Simpson, R.B, Edwards, J.F, Anders, D.R, Thomson, T.D
Format: Article
Language:English
Subjects:
Animals
antibacterial properties
Antitubercular Agents - pharmacology
Antitubercular Agents - therapeutic use
Cattle
cattle diseases
Cattle Diseases - drug therapy
Cattle Diseases - microbiology
Disease Models, Animal
Drug Resistance, Bacterial
Female
macrolide antibiotics
Macrolides - pharmacology
Macrolides - therapeutic use
Mice
Mice, Inbred C57BL
Microbial Sensitivity Tests
Monensin - pharmacology
Monensin - therapeutic use
Mycobacterium avium subsp. paratuberculosis - drug effects
Mycobacterium avium subsp. paratuberculosis - pathogenicity
Paratuberculosis - drug therapy
Paratuberculosis - microbiology
Tylosin - analogs & derivatives
Tylosin - pharmacology
Tylosin - therapeutic use
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Summary:This study was designed to determine the susceptibility in vitro and infectivity of 1 field isolate of Mycobacterium avium sbsp paratuberculosis after exposure to monensin sodium and tilmicosin phosphate. Minimum inhibitory concentrations (0.39 microg monensin sodium/mL; 1.60 microg tilmicosin phosphate/mL) were determined in quintuplicate. Organisms were then incubated with 3 different concentrations of each medication for 3 different lengths of time, then washed and resuspended in sterile physiologic saline and injected intraperitoneally into mice that were genetically susceptible to infection. Mice were euthanatized 50 d later and the number of hepatic granulomas was used as the indicator of infectivity. Neither time of incubation nor concentration of medication had any effect on the infectivity of the organisms. Monensin sodium significantly reduced the number of hepatic granulomas in genetically susceptible mice while tilmicosin phosphate did not. Antimycobacterial activity of monensin sodium suggests that the role of monensin in the control of bovine paratuberculosis should be evaluated further.
ISSN:0830-9000