The Role of NK and T Cells in Endometriosis

• Published in: International journal of molecular sciences 2024-09, Vol.25 (18), p.10141
• Main Authors: Reis, José Lourenço, Rosa, Natacha Nurdine, Martins, Catarina, Ângelo-Dias, Miguel, Borrego, Luís Miguel, Lima, Jorge
• Format: Article
• Language: English
• Subjects: Adult; Age; Ascitic Fluid - immunology; Ascitic Fluid - metabolism; Ascitic Fluid - pathology; Body mass index; CD56 Antigen - metabolism; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; Cells; Complications and side effects; Cytotoxicity; Development and progression; Endometriosis; Endometriosis - immunology; Endometriosis - metabolism; Endometriosis - pathology; Female; Flow Cytometry; Health aspects; Humans; Immunology; Infertility; Killer cells; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; Lymphocytes; Pathophysiology; Physiological aspects; Sexual disorders; T cells; T-Lymphocytes - immunology; T-Lymphocytes - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms251810141

Endometriosis, a debilitating condition, affects one in ten women of reproductive age. Its pathophysiology remains unclear, though deficiencies in immune surveillance are thought to create an environment conducive to the evasion of ectopic endometrial cells from the immune system. Our research explores the immunological impact of endometriosis both locally and systemically, emphasizing natural killer (NK) and T cell subpopulations. We incorporated 62 female patients who underwent laparoscopic surgery; of those, 47 had endometriosis, and 15 were controls. We collected peritoneal fluid (PF) and peripheral blood (PB) samples which were tagged with monoclonal antibodies and subsequently scrutinized using flow cytometry. Our findings revealed significant differences in immunological profiles based on demographic factors and symptomatology. In the endometriosis cohort, there was an increase in PB CD56 CD16 and PF CD8 CD56 CD16 NK cells. CD16 CD4 T cell levels were significantly lower in the PB of endometriosis patients who smoke. Individuals with more severe disease displayed significantly higher levels of PB CD16 CD8 T cells, which also increased in those with non-menstrual pelvic pain. Dysmenorrhea severity correlated with a progressive increase in PF CD8 CD56 CD16 NK cells. These variations in specific lymphocyte subsets, namely, within NK and T cells, suggest potential immunological mechanisms in the evolution and clinical presentation of endometriosis.