Analyzing the Spatial Distribution of Immune Cells in Lung Adenocarcinoma

(1) Background: This study investigates the tumor immune microenvironment, focusing on immune cell distribution in lung adenocarcinoma. (2) Methods: We evaluated fifty cases of lung adenocarcinoma, and suitable areas for further studies were annotated on the histological slides. Two tumor cores per...

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Bibliographic Details
Published in:Journal of personalized medicine 2024-08, Vol.14 (9), p.925
Main Authors: Almarii, Florina, Sajin, Maria, Simion, George, Dima, Simona O, Herlea, Vlad
Format: Article
Language:English
Subjects:
Adenocarcinoma
B cells
CD163 antigen
CD4 antigen
CD8 antigen
Cells
Cloning
Cytotoxicity
Development and progression
Immune system
Immunohistochemistry
Immunotherapy
Localization
Lung cancer
Lymphocytes
Lymphocytes T
Macrophages
Medical prognosis
Microenvironments
Patients
PD-L1 protein
Software
Spatial distribution
Spatial heterogeneity
Statistical analysis
T cells
Tumor cells
Tumors
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Summary:(1) Background: This study investigates the tumor immune microenvironment, focusing on immune cell distribution in lung adenocarcinoma. (2) Methods: We evaluated fifty cases of lung adenocarcinoma, and suitable areas for further studies were annotated on the histological slides. Two tumor cores per case were obtained, one from the tumor's center and another from its periphery, and introduced into three paraffin receptor blocks for optimized processing efficiency. The 4-micrometer-thick tissue microarray sections were stained for H&E and for CD68, CD163, CD8, CD4, and PD-L1; (3) Results: Our investigation revealed significant correlations between PD-L1 expression in tumor cells and the presence of CD163+ macrophages, between CD4+ cells and CD8+, CD68+, and CD163+ cells, and also between CD8+ T cells and CD163+ cells. Additionally, while we observed some differences in cellular components and densities between the tumor center and periphery, these differences were not statistically significant. However, distinct correlations between PD-L1 and immune cells in these regions were identified, suggesting spatial heterogeneity in the immune landscape. (4) Conclusions: These results emphasize the intricate interactions between immune cells and tumor cells in lung adenocarcinoma. Understanding patient spatial immune profile could improve patient selection for immunotherapy, ensuring that those most likely to benefit are identified.
ISSN:2075-4426
2075-4426
DOI:10.3390/jpm14090925