Variant chromatin structure of the oriP region of Epstein-Barr virus and regulation of EBER1 expression by upstream sequences and oriP

Most of the Epstein-Barr virus genome in latently infected cells is in a standard nucleosomal structure, but the region encompassing oriP and the Epstein-Barr virus-encoded small RNA (EBER) genes shows a distinctive pattern when digested with micrococcal nuclease. This pattern corresponds to a previ...

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Bibliographic Details
Published in:Journal of virology 2001-07, Vol.75 (13), p.6235-6241
Main Authors: Wensing, B, Stühler, A, Jenkins, P, Hollyoake, M, Karstegl, C E, Farrell, P J
Format: Article
Language:English
Subjects:
Chromatin - chemistry
DNA Replication
DNA, Viral - metabolism
EBER1 gene
Epstein-Barr virus
Gene Expression Regulation, Viral
Herpesvirus 4, Human - genetics
Promoter Regions, Genetic
RNA, Viral - genetics
Transcription, Genetic
Virus-Cell Interactions
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Summary:Most of the Epstein-Barr virus genome in latently infected cells is in a standard nucleosomal structure, but the region encompassing oriP and the Epstein-Barr virus-encoded small RNA (EBER) genes shows a distinctive pattern when digested with micrococcal nuclease. This pattern corresponds to a previously mapped nuclear matrix attachment region. Although the EBER genes are adjacent to oriP, there is only a two- to fourfold effect of oriP on EBER expression. However, sequences containing a consensus ATF site upstream of EBER1 are important for EBER1 expression.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.75.13.6235-6241.2001