Deglycosylation by trifluoromethanesulphonic acid of endopeptidase-24.11 purified from pig kidney and intestine

Endopeptidase-24.11, an integral microvillar membrane enzyme, exists in differently glycosylated forms when purified from pig kidney and intestine [Fulcher, Chaplin & Kenny (1983) Biochem. J. 215, 317-323]. When these glycoproteins, and another form of the kidney enzyme prepared from the Yucatan...

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Bibliographic Details
Published in:Biochemical journal 1984-08, Vol.221 (3), p.919-922
Main Authors: Stewart, J.R, Chaplin, M.F, Kenny, A.J
Format: Article
Language:English
Subjects:
Amino Acids - analysis
animal physiology
Animals
Carbohydrates - analysis
deglycosylation
Electrophoresis, Polyacrylamide Gel
Endopeptidases
intestine
Intestines - enzymology
kidney
Kidney - enzymology
kidney brush border neutral proteinase
Mesylates
Neprilysin
pigs
Species Specificity
Swine
trifluoromethanesulphonic acid
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Summary:Endopeptidase-24.11, an integral microvillar membrane enzyme, exists in differently glycosylated forms when purified from pig kidney and intestine [Fulcher, Chaplin & Kenny (1983) Biochem. J. 215, 317-323]. When these glycoproteins, and another form of the kidney enzyme prepared from the Yucatan dwarf strain of piglet, were treated, under controlled conditions, with trifluoromethanesulphonic acid, the proteins were freed of carbohydrate and all had the same apparent subunit Mr (77 000) even though the untreated forms varied from Mr 89 000 to Mr 95 000.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2210919