Pertussis toxin blocks the inhibitory effect of muscarinic cholinergic agonists on cyclic AMP accumulation and prolactin secretion in GH3 anterior-pituitary tumour cells

The inhibition of prolactin secretion and cyclic AMP accumulation in GH3 cells by muscarinic agonists was blocked by preincubation of the cells with pertussis toxin (islet-activating protein). There was a lag of approx. 80 min in the onset of the effect on secretion. These results suggest that musca...

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Bibliographic Details
Published in:Biochemical journal 1984-10, Vol.223 (1), p.145-149
Main Authors: Brown, B L, Wojcikiewicz, R J, Dobson, P R, Robinson, A, Irons, L I
Format: Article
Language:English
Subjects:
Adenylate Cyclase Toxin
Animals
Bacterial Toxins - pharmacology
Cell Line
Cyclic AMP - metabolism
Oxotremorine - pharmacology
Parasympathomimetics - pharmacology
Pertussis Toxin
Pituitary Gland, Anterior - metabolism
Pituitary Neoplasms - metabolism
Prolactin - metabolism
Rats
Vasoactive Intestinal Peptide - pharmacology
Virulence Factors, Bordetella
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Summary:The inhibition of prolactin secretion and cyclic AMP accumulation in GH3 cells by muscarinic agonists was blocked by preincubation of the cells with pertussis toxin (islet-activating protein). There was a lag of approx. 80 min in the onset of the effect on secretion. These results suggest that muscarinic agonists decrease prolactin secretion by inhibiting adenylate cyclase activity.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2230145