Isolation of rat mammary epithelial cells for polyamine analysis

Mammary epithelial cells were isolated from either abdominal-inguinal glands or mammary tumours of rats, after enzymic digestion of the tissues, and were analysed for polyamine content. Optimum conditions were developed for the isolation of cells in sufficient yield for the analysis of polyamines fr...

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Bibliographic Details
Published in:Biochemical journal 1984-11, Vol.223 (3), p.929-932
Main Authors: Minocha, R, Thompson, H J, Meeker, L D, Herbst, E J
Format: Article
Language:English
Subjects:
Animals
Cell Separation - methods
DNA - metabolism
Epithelial Cells
Epithelium - metabolism
Female
In Vitro Techniques
Mammary Glands, Animal - cytology
Mammary Glands, Animal - metabolism
Mammary Neoplasms, Experimental - metabolism
Polyamines - metabolism
Rats
Rats, Inbred Strains
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Summary:Mammary epithelial cells were isolated from either abdominal-inguinal glands or mammary tumours of rats, after enzymic digestion of the tissues, and were analysed for polyamine content. Optimum conditions were developed for the isolation of cells in sufficient yield for the analysis of polyamines from 1 g of mammary gland or 0.5 g of tumour tissue. Complete recoveries of the polyamines in the tissues were achieved in the isolated epithelial cells.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2230929