The relationship between pertussis-toxin-induced ADP-ribosylation of a plasma-membrane protein and reversal of muscarinic inhibition of prolactin secretion in GH3 cells

Pertussis toxin (PT) caused the ADP-ribosylation of a Mr-41 000 protein in GH3-cell plasma-membrane preparations. This effect, and muscarinic inhibition of prolactin release, were reversed at similar rates by pretreatment of intact cells with PT. These results suggest that the Mr-41 000 protein is m...

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Bibliographic Details
Published in:Biochemical journal 1984-11, Vol.224 (1), p.339-342
Main Authors: Wojcikiewicz, R J, Dobson, P R, Irons, L I, Robinson, A, Brown, B L
Format: Article
Language:English
Subjects:
Adenosine Diphosphate Ribose - metabolism
Adenylate Cyclase Toxin
Animals
Autoradiography
Bacterial Toxins - pharmacology
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Membrane Proteins - metabolism
Nucleoside Diphosphate Sugars - metabolism
Oxotremorine - pharmacology
Pertussis Toxin
Pituitary Neoplasms - metabolism
Prolactin - metabolism
Rats
Virulence Factors, Bordetella
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Summary:Pertussis toxin (PT) caused the ADP-ribosylation of a Mr-41 000 protein in GH3-cell plasma-membrane preparations. This effect, and muscarinic inhibition of prolactin release, were reversed at similar rates by pretreatment of intact cells with PT. These results suggest that the Mr-41 000 protein is modified in intact GH3 cells, and that this protein (a component of the putative Ni unit of adenylate cyclase) is involved in the expression of muscarinic inhibition.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2240339