ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests

An ELISA method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti human IgG-horse radish peroxidase conjugate as an enzymatic tracer. In order to assess ELISA sensitivity and specificity, a between methods c...

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Bibliographic Details
Published in:Journal of clinical pathology 1980-07, Vol.33 (7), p.640-643
Main Authors: Balsari, A, Poli, G, Molina, V, Dovis, M, Petruzzelli, E, Boniolo, A, Rolleri, E
Format: Article
Language:English
Subjects:
Antibodies - analysis
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G - analysis
Serologic Tests - methods
Toxoplasma - immunology
Toxoplasmosis - diagnosis
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Summary:An ELISA method was developed for the measurement of toxoplasma IgG antibodies in human serum using antigen-coated polystyrene beads as a solid phase and anti human IgG-horse radish peroxidase conjugate as an enzymatic tracer. In order to assess ELISA sensitivity and specificity, a between methods comparison was made using 'conventional' serological tests as reference (dye-test, crossover-linked immunoassay, passive haemagglutination, indirect immunofluorescence). From an analysis of the group classifications obtained some considerations emerged: the ELISA specificity looks comparable with that of the 'reference' tests, as no sample classified as negative by all these tests was ELISA-positive, and vice versa; ELISA appears to correlate better with haemagglutination and immunofluorescence, on the basis of the respective class frequencies; in particular, the number of positives, which is much lower for the dye-test and crossover-linked immunoassay, suggests that a higher sensitivity is reached in the former cases.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.33.7.640