The transport of glutamine and alanine into rat colonocytes

The transport of glutamine and alanine into isolated rat colonocytes was studied. The transport of both amino acids appears to be dependent on a Na+ gradient. The apparent Km values for the transport of glutamine and alanine were 2.56 +/- 0.84 and 5.35 +/- 1.20 mM respectively, but with similar Vmax...

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Bibliographic Details
Published in:Biochemical journal 1986-08, Vol.238 (1), p.131-135
Main Author: Ardawi, M S
Format: Article
Language:English
Subjects:
alanine
Alanine - metabolism
Amino Acids - pharmacology
Animals
Biological Transport - drug effects
Colon - metabolism
colonocytes
Energy Metabolism
glutamine
Glutamine - metabolism
In Vitro Techniques
Kinetics
Male
Rats
Rats, Inbred Strains
Sodium - pharmacology
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Summary:The transport of glutamine and alanine into isolated rat colonocytes was studied. The transport of both amino acids appears to be dependent on a Na+ gradient. The apparent Km values for the transport of glutamine and alanine were 2.56 +/- 0.84 and 5.35 +/- 1.20 mM respectively, but with similar Vmax. values. Glutamine and alanine transport were mutually competitive, and the transport of both amino acids was competitively inhibited by 2-methylaminoisobutyrate. In contrast, histidine inhibited the transport of both glutamine and alanine non-competitively. It is concluded that glutamine and alanine are transported into rat colonocytes by a common carrier system similar to System A of other cells. It is suggested that the metabolic function of this transport system in rat colonocytes might be the partial exchange of extracellular glutamine for intracellular alanine.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2380131