Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum)

A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a be...

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Bibliographic Details
Published in:Biochemical journal 1987-05, Vol.243 (3), p.723-728
Main Authors: Ramadoss, C.S, Shenoy, B.C, Borthakur, A
Format: Article
Language:English
Subjects:
characterization
chloroplasts
Chromatography, Liquid
Cicer arietinum
cytochromes
Electrophoresis, Polyacrylamide Gel
haemoprotein b-559
Heme - analysis
Hemeproteins - isolation & purification
isolation
photosynthesis
plant proteins
Seeds - analysis
Seeds - enzymology
Spectrophotometry
Ultracentrifugation
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Summary:A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a beta-band at 530 nm. In the reduced state the Bengal-gram haemoprotein showed reactivity towards CO, nitrite and hydroxylamine. SDS/polyacrylamide-slab-gel electrophoresis showed that the haemoprotein has Mr 78,000. Gel-filtration and ultracentrifugal analyses suggest that the Bengal-gram haemoprotein is oligomeric in nature. Since it differs from photosynthetic membrane cytochrome b-559 in solubility in buffer, in reactivity towards CO and in molecular size, it appears to be a novel haemoprotein b-559.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2430723