The Potential of QP3VH-Chitosan Peptide as Biomimetic Remineralization in Early Dental Caries Treatment: An In Vitro Study

• Published in: European journal of dentistry 2024-10, Vol.18 (4), p.1149-1156
• Main Authors: Agusmawanti, Prima, Ratih, Diatri Nari, Purwanti, Nunuk, Raharjo, Tri Joko
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 1305-7456; 1305-7464
• DOI: 10.1055/s-0044-1782189

The development of remineralization biomimetics using organic peptide molecules is expected to resemble the hydroxyapatite (HA) mineralization process in tooth enamel. The development of an amelogenin derivative peptide combined with antimicrobial peptide was designed, resulting in QP3VH. This combination then was mixed with chitosan as a carrier. This study aimed to evaluate the biomimetic efficacy of QP3VH as a remineralizing agent combined with chitosan.  Fifty deciduous mandibular incisor enamel samples were used in this study. The artificial enamel lesions were created on a buccal surface and were randomly assigned to five groups of 10 each according to the remineralizing agent used: QP3VH, NaF, QP3VH + NaF, QP3VH + CS (QP3VH + chitosan), and saline distilled water (SDW). Each group was performed pH cycling for seven days. Enamel surface morphology and evaluation of mineral content Ca/P (calcium and phosphate) using scanning electron microscopy and energy dispersive X-ray analysis. The assessment was carried out, after demineralization, and after application with remineralization agents.  Data were analyzed using a one-way analysis of variance followed by least significance difference post-hoc test. The paired -test was utilized to compare the demineralization and remineralization results. The significance level used was 95%.  The remineralized group exhibited a significant increase in calcium and phosphate content on the enamel surface (