Phorbol-ester-induced alterations of free calcium ion transients in single rat hepatocytes

The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series o...

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Bibliographic Details
Published in:Biochemical journal 1987-09, Vol.246 (3), p.619-623
Main Authors: Woods, N M, Cuthbertson, K S, Cobbold, P H
Format: Article
Language:English
Subjects:
Aequorin
Animals
Arginine Vasopressin - pharmacology
Biological Transport - drug effects
calcium
Calcium - pharmacokinetics
hepatocytes
In Vitro Techniques
Liver - drug effects
Liver - metabolism
Male
Phenylephrine - pharmacology
Phorbol 12,13-Dibutyrate
phorbol esters
Phorbol Esters - pharmacology
Rats
Rats, Inbred Strains
Tetradecanoylphorbol Acetate - pharmacology
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Summary:The effect of the phorbol esters phorbol 12-myristate 13-acetate (TPA) and phorbol 12,13-dibutyrate (PDB) on changes in free Ca2+ concentration ([Ca2+]i) in single rat hepatocytes, microinjected with the photoprotein aequorin, were investigated. [Arg8]vasopressin and phenylephrine induced a series of repetitive [Ca2+]i transients. Phorbol esters inhibited the alpha 1-adrenoceptor-induced response; sub-nanomolar concentrations decreased the transient frequency, and higher concentrations abolished the transients. The inhibitory effect of PDB was readily reversible. Phorbol esters were less effective in decreasing the frequency of [Arg8]-vasopressin-induced transients, and the inhibition could be overcome by high [Arg8]vasopressin concentrations.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2460619