Fluorescence and bioluminescence measurement of cytoplasmic free calcium

One development has been the refinement of the use of the calcium sensitive photoproteins aequorin and obelin. Part of this review is devoted to an explanation of this method, its advantages and pitfalls, and its successes. This review covers the technical considerations of using fluorescent and bio...

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Bibliographic Details
Published in:Biochemical journal 1987-12, Vol.248 (2), p.313-328
Main Authors: Cobbold, P H, Rink, T J
Format: Article
Language:English
Subjects:
Animals
Calcium - analysis
Cytoplasm - analysis
Fluorescent Dyes
Fluorometry
Luminescent Measurements
Luminescent Proteins
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Summary:One development has been the refinement of the use of the calcium sensitive photoproteins aequorin and obelin. Part of this review is devoted to an explanation of this method, its advantages and pitfalls, and its successes. This review covers the technical considerations of using fluorescent and bioluminescent probes for measuring (Ca super(2+)) sub(i).
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2480313