Effects of chronic insecticide exposure on neuronal network development in vitro in rat cortical cultures

• Published in: Archives of toxicology 2024-11, Vol.98 (11), p.3837-3857
• Main Authors: van Melis, Lennart V. J., Peerdeman, Anneloes M., González, Celia Arenas, van Kleef, Regina G. D. M., Wopken, J. Pepijn, Westerink, Remco H. S.
• Format: Article
• Language: English
• Subjects: Aldicarb; Animals; Benzoic acid; Biomedical and Life Sciences; Biomedicine; Carbamate pesticides; Carbamates (tradename); Carbamates - toxicity; Carbaryl; Cells, Cultured; Cerebral Cortex - drug effects; Chlorpyrifos; Chronic effects; Chronic exposure; Cypermethrin; Environmental Health; Exposure; Female; Females; Firing pattern; Insecticides; Insecticides - toxicity; Male; Males; Microelectrodes; Nerve Net - drug effects; Neural networks; Neurodevelopment; Neurons - drug effects; Neurotoxicity; Occupational Medicine/Industrial Medicine; Organ Toxicity and Mechanisms; Organophosphates; Permethrin; Pesticides; Pharmacology/Toxicology; Pyrethrins - toxicity; Pyrethroids; Rats; Rats, Wistar; Sex; Sex differences; Sex Factors
• ISSN: 0340-5761; 1432-0738; 1432-0738
• DOI: 10.1007/s00204-024-03840-0

Developmental exposure to carbamates, organophosphates, and pyrethroids has been associated with impaired neurodevelopmental outcomes. Sex-specific differences following chronic insecticide exposure are rather common in vivo. Therefore, we assessed the chronic effects of in vitro exposure to different carbamates (carbaryl, methomyl and aldicarb), organophosphates [chlorpyrifos (CPF), chlorpyrifos-oxon (CPO), and 3,5,6,trichloropyridinol (TCP)], and pyrethroids [permethrin, alpha-cypermethrin and 3-phenoxy benzoic acid (3-PBA)] on neuronal network development in sex-separated rat primary cortical cultures using micro-electrode array (MEA) recordings. Our results indicate that exposure for 1 week to carbaryl inhibited neurodevelopment in male cultures, while a hyperexcitation was observed in female cultures. Methomyl and aldicarb evoked a hyperexcitation after 2 weeks of exposure, which was more pronounced in female cultures. In contrast to acute MEA results, exposure to ≥ 10 µM CPF caused hyperexcitation in both sexes after 10 days. Interestingly, exposure to 10 µM CPO induced a clear hyperexcitation after 10 days of exposure in male but not female cultures. Exposure to 100 µM CPO strongly inhibited neuronal development. Exposure to the type I pyrethroid permethrin resulted in a hyperexcitation at 10 µM and a decrease in neuronal development at 100 µM. In comparison, exposure to ≥ 10 µM of the type II pyrethroid alpha-cypermethrin decreased neuronal development. In female but not in male cultures, exposure to 1 and 10 µM permethrin changed (network) burst patterns, with female cultures having shorter (network) bursts with fewer spikes per (network) burst. Together, these results show that MEA recordings are suitable for measuring sex-specific developmental neurotoxicity in vitro. Additionally, pyrethroid exposure induced effects on neuronal network development at human-relevant concentrations. Finally, chronic exposure has different effects on neuronal functioning compared to acute exposure, highlighting the value of both exposure paradigms.