Potential application of a combined extract of Channa striata and calcium hydroxide for inhibiting lymphocytes and interleukin-1β cells in the asymptomatic irreversible pulpitis of Wistar rats ( Rattus norvegicus )

• Published in: Journal of conservative dentistry 2024-09, Vol.27 (9), p.942-948
• Main Authors: Nugroho, Juni Jekti, Taha, Risnawati, Trilaksana, Aries Chandra, Dwiandhany, Wahyuni Suci
• Format: Article
• Language: English
• Subjects: Ethylenediaminetetraacetic acid; Hydroxides; Inflammation; Interleukins; Lymphocytes; Original; Pancreatic beta cells
• ISSN: 2950-4716; 0972-0707; 2950-4708; 2950-4708
• DOI: 10.4103/JCDE.JCDE_407_24

This study aimed to evaluate the effectiveness of combining extract with calcium hydroxide (Ca(OH)2) in reducing lymphocyte counts and interleukin (IL)-1β levels in inflamed pulp teeth. A laboratory experimental setup with a posttest control group (CG) design was employed. Thirty-two rat incisor teeth were divided into four groups: a positive CG (100% Ca(OH)2), treatment 1 (25% extract + 75% Ca(OH)2), treatment 2 (50% extract + 50% Ca(OH)2), and treatment 3 (75% extract + 25% Ca(OH)2). These test materials were applied to Class 1 cavities extending to the pulp roof, with samples collected on days 1, 3, 5, and 7. Data were analyzed using analysis of variance and least significant difference tests. In all the groups, the highest levels of lymphocytes and IL-1β were observed on day 3, followed by a decline on days 5 and 7. The combination of extract with Ca(OH)2 significantly reduced inflammatory markers, particularly lymphocyte and IL-1β levels. The extract of holds significant potential as an innovative therapeutic approach for managing inflamed dental pulp.