Evaluation of Automatic Signal Detection of In Situ Hybridization for Detecting HPV DNA in Cervical Tissue Derived from Patients with Cervical Intraepithelial Neoplasia

• Published in: Cancers 2024-10, Vol.16 (20), p.3485
• Main Authors: Przybylski, Marcin, Millert-Kalińska, Sonja, de Mezer, Mateusz, Krzyżaniak, Monika, Kurzawa, Paweł, Żurawski, Jakub, Jach, Robert, Pruski, Dominik
• Format: Article
• Language: English
• Subjects: Biopsy; Cancer research; Cellular biology; Cervical cancer; Cervix; Cervix dysplasia; Deoxyribonucleic acid; Diagnosis; DNA; Enzymes; Genetic aspects; Genetic screening; Genetic testing; Genotyping; Health aspects; Human papillomavirus; Hybridization; Immunohistochemistry; Iodine; Laboratories; Medical practices; Medical research; Methods; Morphology; Papillomavirus infections; Patients; Polymerase chain reaction; Sensitivity analysis; Software; Womens health
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers16203485

Cervical cancer is fourth the most common cancer in women worldwide. Due to the prevalence of human papillomavirus (HPV) in the population (80-90%), scientists are likely to discover even more associations of this pathogen with other diseases in the future. In recent years, In Situ Hybridization (ISH) assays that use automated signal-detecting methods in formalin-fixed, paraffin-embedded (FFPE) cervical tissue, such as the enzyme-categorized signal-detecting system, have shown a higher sensitivity. To evaluate automatic signal detection of ISH assay for detecting HPV DNA, we compared the ability of an ISH probe, Inform HPV II and III (Ventana Medical Systems, Tucson, AZ), to that of PCR assays to detect HPV DNA in cervical tissue specimens with cervical intraepithelial neoplasia (CIN; CIN 1, 28 cases; CIN 2, 22 cases; and CIN 3, 20 cases) and normal cervix (2 cases). Our findings showed a significant relation was confirmed between ISH III level and HPV outcome (positive/negative). Patients with positive HPV outcomes had significantly lower ISH III levels, MD = -7961.82 CI [-17,230.00; -199.21], = 0.005. Automatic signal detection of ISH assay is not particularly applicable to cervical tissue material. A more useful method of confirming the presence of HPV in the cervix is the HPV test with genotyping, as it allows for collecting a larger amount of material from the cervical disc and canal. The interpretation of a positive or negative ISH test must be guided in the context of clinical history and morphology.