A DNA topoisomerase I inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone

Follicle-stimulating hormone (FSH), acting through a cycle AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effec...

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Bibliographic Details
Published in:Biochemical journal 1991-07, Vol.277 ( Pt 2) (2), p.557-560
Main Authors: Barañao, J L, Bley, M A, Batista, F D, Glikin, G C
Format: Article
Language:English
Subjects:
Animals
Bucladesine - pharmacology
Camptothecin - pharmacology
Cell Differentiation - drug effects
Cells, Cultured
Diethylstilbestrol - pharmacology
DNA Topoisomerases, Type I - metabolism
Female
Follicle Stimulating Hormone - pharmacology
Granulosa Cells - cytology
Granulosa Cells - drug effects
Granulosa Cells - enzymology
Insulin - pharmacology
Kinetics
Methionine - metabolism
Protein Biosynthesis
Rats
Rats, Inbred Strains
Topoisomerase I Inhibitors
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Summary:Follicle-stimulating hormone (FSH), acting through a cycle AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effect was not due to non-specific inhibition of protein synthesis, as shown by measurement of [35S]methionine incorporation. A transient increase in DNA topoisomerase I activity was observed after 24 h of culture in the presence of FSH or dibutyryl cyclic AMP. Our results are consistent with a key role for DNA topoisomerase I in the modulation of gene expression by FSH in rat granulosa cells.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2770557