Carbohydrate structure of human pancreatic elastase 1

Human pancreatic elastase 1 (E1) is a glycoprotein containing two potential N-glycosylation sites, one of which carries a carbohydrate moiety [Wendorf, Geyer, Sziegoleit & Linder (1989) FEBS Lett. 249, 275-278]. In order to study its glycosylation, glycoprotein isolated from post-mortem pancreas...

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Bibliographic Details
Published in:Biochemical journal 1991-09, Vol.278 (2), p.505-514
Main Authors: WENDORF, P, LINDER, D, SZIEGOLEIT, A, GEYER, R
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Glycopeptides - metabolism
Glycosylation
Humans
Hydrogen-Ion Concentration
Hydrolases
Methylation
Molecular Sequence Data
Oligosaccharides - chemistry
Oligosaccharides - metabolism
Pancreas - enzymology
Pancreatic Elastase - chemistry
Pancreatic Elastase - isolation & purification
Sugar Alcohols - chemistry
Trypsin - chemistry
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Summary:Human pancreatic elastase 1 (E1) is a glycoprotein containing two potential N-glycosylation sites, one of which carries a carbohydrate moiety [Wendorf, Geyer, Sziegoleit & Linder (1989) FEBS Lett. 249, 275-278]. In order to study its glycosylation, glycoprotein isolated from post-mortem pancreas tissue of 75 donors was digested with trypsin. Oligosaccharides were liberated from resulting glycopeptides by treatment with peptide-N4-(N-acetyl-beta-glycosaminyl)-asparagine amidase F, radiolabelled by reduction with KB3H4 and separated by h.p.l.c. and gel filtration. Major oligosaccharide alditol fractions, representing 67.8 mol% of total glycans, were characterized by methylation analysis and sequential degradation with exoglycosidases. The results revealed that about two-fifths of the partially truncated, mainly biantennary, complex-type glycans found comprised blood group A, B, Lea (or X), difucosyl A or difucosyl B determinants, which could be assigned to lactosamine antennae linked to Man(alpha 1-3)- residues of the sugar chains.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2780505