Multicenter Evaluation of a Semiautomated, Standardized Assay for Detection of Hepatitis B Virus DNA in Blood Donations

We evaluated the COBAS Ampliscreen hepatitis B virus (HBV) test using standards, seroconversion panels, consecutive donations, and samples from patients with abnormal alanine aminotransferase and chronic hepatitis C. Specificity was 100% and sensitivity was 20 IU/ml. In seroconversion panels, HBV DN...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-06, Vol.43 (6), p.2991-2993
Main Authors: Romanò€, Luisa, Velati, Claudio, Baruffi, Lorella, Fomiatti, Laura, Colucci, Giuseppe, Zanetti, Alessandro R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood Donors
DNA, Viral - blood
Fundamental and applied biological sciences. Psychology
Hepatitis B - diagnosis
Hepatitis B - virology
Hepatitis B Surface Antigens - blood
Hepatitis B virus
Hepatitis B virus - isolation & purification
Humans
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Amplification Techniques - standards
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Virology
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Summary:We evaluated the COBAS Ampliscreen hepatitis B virus (HBV) test using standards, seroconversion panels, consecutive donations, and samples from patients with abnormal alanine aminotransferase and chronic hepatitis C. Specificity was 100% and sensitivity was 20 IU/ml. In seroconversion panels, HBV DNA was detected up to 4 to 18 days before HBsAg, suggesting that this assay is useful in shortening the infectious window phase.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.6.2991-2993.2005