Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells

ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, A...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical journal 1983-07, Vol.214 (1), p.273-276
Main Authors: Pearson, J D, Slakey, L L, Gordon, J L
Format: Article
Language:English
Subjects:
Adenine Nucleotides - pharmacology
Animals
Cells, Cultured
Dose-Response Relationship, Drug
Endothelium - drug effects
Endothelium - metabolism
Epoprostenol - metabolism
Prostaglandins - metabolism
Receptors, Cell Surface - drug effects
Receptors, Purinergic
Stimulation, Chemical
Swine
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, ADP slightly less potent, AMP much less potent and adenosine is ineffective. These results are consistent with the presence of a purinoceptor on endothelium linked to the prostacyclin synthetic pathway.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2140273