Fast-atom-bombardment mass spectrometry and electron-impact direct-probe mass spectrometry in the structural analysis of oligosaccharides with special reference to the poly(N-acetyl-lactosamine) series

A comparison has been made of positive- and negative-ion fast-atom-bombardment (FAB) and electron-impact (EI) mass spectrometry for analysis of oligosaccharides and alditols containing alternating and consecutive sequences of neutral and acetamido sugars. Among these were novel chemically synthesize...

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Bibliographic Details
Published in:Biochemical journal 1984-05, Vol.219 (3), p.947-952
Main Authors: Hounsell, E F, Madigan, M J, Lawson, A M
Format: Article
Language:English
Subjects:
Acetamides - analysis
Carbohydrate Sequence
Chemical Phenomena
Chemistry
Electrons
Hexoses - analysis
Mass Spectrometry
Oligosaccharides
Polysaccharides
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Summary:A comparison has been made of positive- and negative-ion fast-atom-bombardment (FAB) and electron-impact (EI) mass spectrometry for analysis of oligosaccharides and alditols containing alternating and consecutive sequences of neutral and acetamido sugars. Among these were novel chemically synthesized tetrasaccharides with Ii antigen activities. FAB ionization has the advantage that it is applicable to non-derivatized oligosaccharides and it can determine Mr. However, the abundance of fragment ions providing structural information and the amount of material required for analysis (1-50 nmol) varied from sample to sample. In contrast, EI mass spectrometry of 5 nmol of permethylated or peracetylated oligosaccharides reliably gives all the fragment ions formed by cleavage across the glycosidic bonds.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2190947