Regulation of a single inositol 1‐phosphate synthase homeologue by HSFA6B contributes to fibre yield maintenance under drought conditions in upland cotton

• Published in: Plant biotechnology journal 2024-10, Vol.22 (10), p.2756-2772
• Main Authors: Yu, Li'ang, Dittrich, Anna C. Nelson, Zhang, Xiaodan, Brock, Jordan R., Thirumalaikumar, Venkatesh P., Melandri, Giovanni, Skirycz, Aleksandra, Edger, Patrick P., Thorp, Kelly R., Hinze, Lori, Pauli, Duke, Nelson, Andrew D.L.
• Format: Article
• Language: English
• Subjects: Agricultural production; Arabidopsis; Binding sites; Climate change; Cotton; Cotton Fiber; cotton fibre yield; Crop yield; Cultivars; DNA sequencing; Drought; drought response; Droughts; Gene Expression Regulation, Plant; gene regulatory network; Gene sequencing; Genes; Genome-Wide Association Study; Genomes; Genotype & phenotype; Gossypium - genetics; Gossypium - metabolism; Gossypium hirsutum; Inositols; Metabolism; Metabolites; metabolomics; phenomics; Phylogenetics; Phylogeny; Physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Regulatory mechanisms (biology); Single-nucleotide polymorphism; Stress, Physiological - genetics; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptomes; transcriptomics; Vegetation; Water shortages
• ISSN: 1467-7644; 1467-7652; 1467-7652
• DOI: 10.1111/pbi.14402

Summary Drought stress substantially impacts crop physiology resulting in alteration of growth and productivity. Understanding the genetic and molecular crosstalk between stress responses and agronomically important traits such as fibre yield is particularly complicated in the allopolyploid species, upland cotton (Gossypium hirsutum), due to reduced sequence variability between A and D subgenomes. To better understand how drought stress impacts yield, the transcriptomes of 22 genetically and phenotypically diverse upland cotton accessions grown under well‐watered and water‐limited conditions in the Arizona low desert were sequenced. Gene co‐expression analyses were performed, uncovering a group of stress response genes, in particular transcription factors GhDREB2A‐A and GhHSFA6B‐D, associated with improved yield under water‐limited conditions in an ABA‐independent manner. DNA affinity purification sequencing (DAP‐seq), as well as public cistrome data from Arabidopsis, were used to identify targets of these two TFs. Among these targets were two lint yield‐associated genes previously identified through genome‐wide association studies (GWAS)‐based approaches, GhABP‐D and GhIPS1‐A. Biochemical and phylogenetic approaches were used to determine that GhIPS1‐A is positively regulated by GhHSFA6B‐D, and that this regulatory mechanism is specific to Gossypium spp. containing the A (old world) genome. Finally, an SNP was identified within the GhHSFA6B‐D binding site in GhIPS1‐A that is positively associated with yield under water‐limiting conditions. These data lay out a regulatory connection between abiotic stress and fibre yield in cotton that appears conserved in other systems such as Arabidopsis.