Supraspinatus Muscle Regeneration Following Rotator Cuff Tear: A Study of the Biomarkers Pax7, MyoD, and Myogenin

• Published in: International journal of molecular sciences 2024-11, Vol.25 (21), p.11742
• Main Authors: Hejbøl, Eva Kildall, Andkjær, Stephanie Wej, Dybdal, Julie, Klindt, Marie, Möller, Sören, Lambertsen, Kate Lykke, Schrøder, Henrik Daa, Frich, Lars Henrik
• Format: Article
• Language: English
• Subjects: Adult; Age; Aged; Atrophy; Back; Biomarkers - metabolism; Biopsy; Care and treatment; Cell cycle; Female; Gender differences; Humans; Male; Middle Aged; Muscle Development; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; MyoD Protein - metabolism; Myogenesis; Myogenin - metabolism; Patients; PAX7 Transcription Factor - metabolism; Regeneration; Rotator cuff; Rotator Cuff - metabolism; Rotator Cuff - pathology; Rotator Cuff Injuries - metabolism; Rotator Cuff Injuries - pathology; Rotator Cuff Injuries - surgery; Shoulder injuries; Stem cells; Trauma
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms252111742

The success of rotator cuff tendon repair relies on both tendon healing and muscle recovery. The objective of this descriptive study was to investigate the regenerative potential of the supraspinatus muscle in rotator cuff tear conditions by quantifying the expression of Pax7, MyoD, and myogenin, basic factors that regulate myogenesis. Muscle biopsies were collected from thirty-three patients aged 34 to 73 years who underwent surgery for a rotator cuff tear affecting the supraspinatus muscle. Among these patients, twenty-seven percent were women, and the age of the lesions ranged from 2 to 72 months post-initial trauma. Biopsies were harvested from the supraspinatus muscle at the end closest to the tendon, and control biopsies were harvested from the ipsilateral deltoid muscle. The densities of immunohistochemically stained Pax7 , MyoD , and myogenin nuclei/mm were used to estimate the myogenic potential of the muscle. Adjustments were made for patient age and lesion age. We found increased density of MyoD and myogenin cells in supraspinatus muscles compared to deltoid muscles ( < 0.001 and = 0.003, respectively). Regression analyses that combined the density of positive nuclei with patient age showed a continuous increase in Pax7 with age but also a reduction of MyoD and myogenin in older patients. When combined with lesion age, there was a decline in the density of all myogenic markers after an initial rise. Pax7 density continued to be higher in supraspinatus compared to the deltoid muscle, but the density of MyoD and myogenin terminally dropped to a density lower than in the deltoid. Our findings suggest that the supraspinatus muscle in tear conditions showed signs of initial activation of muscle regeneration. When compared to the unaffected deltoid muscle, an apparent reduction in capacity to progress to full muscle fiber maturity was also demonstrated. This pattern of inhibited myogenesis seemed to increase with both patient age and lesion age. Our results on muscle regenerative capacity indicate that younger patients with rotator cuff tears have better chances of muscle recovery and may benefit from early surgical reconstruction.