A Comparison of Molecular Techniques for Improving the Methodology in the Laboratory of Pharmacogenetics

One of the most critical goals in healthcare is safe and effective drug therapy, which is directly related to an individual's response to treatment. Precision medicine can improve drug safety in many scenarios, including polypharmacy, and it requires the development of new genetic characterizat...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-11, Vol.25 (21), p.11505
Main Authors: Peña-Martín, María Celsa, Marcos-Vadillo, Elena, García-Berrocal, Belén, Heredero-Jung, David Hansoe, García-Salgado, María Jesús, Lorenzo-Hernández, Sandra Milagros, Larrue, Romain, Lenski, Marie, Drevin, Guillaume, Sanz, Catalina, Isidoro-García, María
Format: Article
Language:English
Subjects:
Child & adolescent psychiatry
Clinical medicine
Clopidogrel
Comparative analysis
Drug interactions
Enzymes
Genes
Genetic aspects
Genetic engineering
Genotype & phenotype
Humans
Laboratories
Mass spectrometry
Metabolism
Methods
Patients
Pharmacogenetics
Pharmacogenetics - methods
Polypharmacy
Precision medicine
Precision Medicine - methods
Real-Time Polymerase Chain Reaction - methods
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:One of the most critical goals in healthcare is safe and effective drug therapy, which is directly related to an individual's response to treatment. Precision medicine can improve drug safety in many scenarios, including polypharmacy, and it requires the development of new genetic characterization methods. In this report, we use real-time PCR, microarray techniques, and mass spectrometry (MALDI-TOF), which allows us to compare them and identify the potential benefits of technological improvements, leading to better quality medical care. These comparative studies, as part of our pharmacogenetic Five-Step Precision Medicine (5SPM) approach, reveal the superiority of mass spectrometry over the other methods analyzed and highlight the importance of updating the laboratory's pharmacogenetic methodology to identify new variants with clinical impact.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252111505