Impact of the quality of resected thyroid cancer tissue sample on next‐generation sequencing testing

Activating rearranged during transfection (RET) proto‐oncogene alterations can be identified using next‐generation sequencing (NGS) of tumor DNA/RNA. We assessed factors associated with NGS (Oncomine Dx Target Test [ODxTT]) success for resected thyroid cancer (TC) specimens, including sample age, pr...

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Bibliographic Details
Published in:Pathology international 2024-02, Vol.74 (2), p.77-86
Main Authors: Hatanaka, Kanako C., Nakamura, Kenichi, Katoh, Ryohei, Ito, Koichi, Hirokawa, Mitsuyoshi, Miyauchi, Akira, Matsuno, Yoshihiro, Kano, Satoshi, Okada, Yui, Mori, Joji, Ito, Yoichi M., Hatanaka, Yutaka
Format: Article
Language:English
Subjects:
Cancer
Child
Child, Preschool
Deoxyribonucleic acid
DNA
DNA sequencing
DNA, Neoplasm
DNA-directed RNA polymerase
Electrophoresis
Fixatives
High-Throughput Nucleotide Sequencing - methods
high‐throughput DNA/RNA sequencing
Humans
Integrity
Mutation
Nucleic acids
Original
Polymerase chain reaction
Quality assessment
Quality control
Regression analysis
Ret protein
Ribonucleic acid
RNA
RNA processing
Success
Thyroid
Thyroid cancer
thyroid neoplasms
Thyroid Neoplasms - genetics
Thyroid Neoplasms - surgery
tissue fixation
tissue preservation
Transfection
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Summary:Activating rearranged during transfection (RET) proto‐oncogene alterations can be identified using next‐generation sequencing (NGS) of tumor DNA/RNA. We assessed factors associated with NGS (Oncomine Dx Target Test [ODxTT]) success for resected thyroid cancer (TC) specimens, including sample age, processing conditions, and DNA/RNA quality. TC samples were from three Japanese hospitals, with sample age
ISSN:1320-5463
1440-1827
1440-1827
DOI:10.1111/pin.13399