Separation and purification of antioxidant peptides from Idesia polycarpa Maxim. cake meal and study of conformational relationship between them

• Published in: Food science & nutrition 2024-09, Vol.12 (9), p.6206-6225
• Main Authors: Dou, Lei, Zhang, Zimu, Yang, Wenqing, Chen, Yaobing, Luo, Kai, Kan, Jianquan
• Format: Article
• Language: English
• Subjects: AKT protein; AKT1 protein; Amino acids; Antioxidants; Autophagy; Chromatography; Damage assessment; Dextran; Dextrans; Enzymes; Free radicals; Gel chromatography; Gelatinase B; Hepatocellular carcinoma; Hydrogen peroxide; Idesia polycarpa Maxim. cake meal; Kinases; Liver cancer; Mass spectrometry; Mass spectroscopy; Matrix metalloproteinase; Matrix metalloproteinases; Metalloproteinase; Molecular docking; network pharmacology; Oxidative stress; Peptides; Pharmacology; Potassium; Protein purification; Proteins; quantum chemistry; Review; separation and purification; Solid phase methods; Solid phase synthesis; Stat3 protein; Tryptophan; Ultrafiltration
• ISSN: 2048-7177; 2048-7177
• DOI: 10.1002/fsn3.4325

In this study, peptides were isolated and purified from Idesia polycarpa Maxim. cake meal for the first time, with the aim of discovering peptides with excellent antioxidant properties. Peptides were isolated and purified from the cake meal using ultrafiltration and dextran gel chromatography. Fractions with significant antioxidant activity were identified by mass spectrometry (MS) and the peptides were screened and characterized using techniques, such as network pharmacology and molecular docking. The results showed that the CIPs‐I‐F2 fractions possessed excellent antioxidant activities, and a total of seven peptides were screened, with the main targets of action including serine/threonine‐protein kinase AKT (AKT1), signal transducer and activator of transcription 3 (STAT3), and matrix metalloproteinase 9 (MMP9), among which ISKPTWADF had the highest total binding energy to the target. ISKPTWADF was synthesized in vitro by solid‐phase synthesis and showed a dose‐dependent protective effect against the hydrogen peroxide (H2O2)‐induced oxidative damage model in human hepatocellular carcinoma HepG2 cells, with its main active site on the tryptophan indole ring at position 52N‐127H. In this study, peptides were isolated and purified from Idesia polycarpa Maxim. cake meal for the first time, with the aim of discovering peptides with excellent antioxidant properties. Peptides were isolated and purified from the cake meal using ultrafiltration and dextran gel chromatography. Fractions with significant antioxidant activity were identified by mass spectrometry (MS) and the peptides were screened and characterized using techniques, such as network pharmacology and molecular docking.