The pivotal role of ZNF384: driving the malignant behavior of serous ovarian cancer cells via the LIN28B/UBD axis

• Published in: Cell biology and toxicology 2024-11, Vol.40 (1), p.100, Article 100
• Main Authors: Yang, Ye, He, Runze, Li, Dongxiao, Mu, Tianli, Kuang, Ziteng, Wang, Min
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Bioinformatics; Biomarkers; Biomedical and Life Sciences; Cancer; Cell Biology; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cystadenocarcinoma, Serous - genetics; Cystadenocarcinoma, Serous - metabolism; Cystadenocarcinoma, Serous - pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; In vivo methods and tests; Life Sciences; Mice; Mice, Inbred BALB C; Mice, Nude; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Pharmacology/Toxicology; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Transfection; Ubiquitin; Xenotransplantation; Zinc finger proteins
• ISSN: 1573-6822; 0742-2091; 1573-6822
• DOI: 10.1007/s10565-024-09938-6

Zinc finger protein 384 (ZNF384) is a highly conserved transcribed gene associated with the development of multiple tumors, however, its role and mechanism in serous ovarian cancer (SOC) are unknown. We first confirmed that ZNF384 was abnormally highly expressed in SOC tissues by bioinformatics analysis and immunohistochemistry. We further used lentivirus packaging and transfection techniques to construct ZNF384 overexpression or knockdown cell lines, and through a series of cell function experiments, gradually verified that ZNF384 promoted a series of malignant behaviors of SOC cell proliferation, migration, and invasion. By establishing a xenotransplantation model in nude mice, it was confirmed that ZNF384 promoted the progress of SOC in vivo. Mechanistically, Overexpression of ZNF384 enhanced the transcriptional activity of Lin-28 homolog B (LIN28B), which promoted the malignant behavior of SOC cells. In addition, LIN28B could regulate the expression of the downstream factor ubiquitin D (UBD) in SOC cells, further promoting the development of SOC. This study shows that ZNF384 aggravates the malignant behavior of SOC cells through the LIN28B/UBD axis, which may be used as a diagnostic biomarker for patients with SOC. Graphical abstract