Evaluation of placental bed uterine in L-NAME-induced early-onset preeclampsia (EO-PE) like the rat model

Preeclampsia (PE) is the leading cause of maternal death worldwide and is associated with long-term morbidity in both mothers and newborns. Animal modeling is considered a functional source for understanding PE pathogenesis, diagnostic standards, and therapeutic approaches. This study aimed to demon...

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Bibliographic Details
Published in:Turkish journal of obstetrics and gynecology 2024-09, Vol.21 (3), p.180-189
Main Authors: Fitriana, Fitriana, Soetrisno, Soetrisno, Sulistyowati, Sri, Indarto, Dono
Format: Article
Language:English
Subjects:
Clinical Investigation
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Summary:Preeclampsia (PE) is the leading cause of maternal death worldwide and is associated with long-term morbidity in both mothers and newborns. Animal modeling is considered a functional source for understanding PE pathogenesis, diagnostic standards, and therapeutic approaches. This study aimed to demonstrate and evaluate the use of N-nitro-L-arginine methyl ester (L-NAME) in a Wistar rat model under conditions similar to PE. A total of 12 rats were divided into 4 groups, each consisting of 3 members, including the pregnant control group and treatment groups administered low-dose (PE 25 mg/kg L-NAME/day), medium-dose (PE 50 mg/kg L-NAME/day), and high-dose L-NAME (PE 75 mg/kg L-NAME/day) L-NAME from gestational day 4 to 19. Measurements included blood pressure, creatinine, and proteinuria levels, placental histological changes, and placental tissue hypoxia-inducible factor 1-alpha, and plasma endothelial nitric oxide synthase levels. The results showed that intervention with L-NAME at 75 mg/kg body weight/day (PE3) induced PE earlier than that with 50 mg/kg body weight/day L-NAME. The model conditions also support further research into PE pathogenesis.
ISSN:2149-9322
2149-9330
DOI:10.4274/tjod.galenos.2024.99132