Development of a Novel Stress and Immune Gene Panel for the Australasian Snapper ( Chrysophrys auratus )

• Published in: Genes 2024-10, Vol.15 (11), p.1390
• Main Authors: Bentley-Hewitt, Kerry L, Flammensbeck, Christina K, Crowhurst, Ross N, Hedderley, Duncan I, Wellenreuther, Maren
• Format: Article
• Language: English
• Subjects: Acclimation; Animal behavior; Animals; Anopheles; Antioxidants; Aquaculture; Aquaculture industry; Biomarkers; Breeding; Cellular stress response; Chrysophrys auratus; Cold; Cooling; Enzymes; Ethylenediaminetetraacetic acid; Experiments; Fish; Fish Proteins - genetics; Fishes; Fishes - genetics; Fishes - immunology; Gene expression; Genes; Genomes; Genotypes; Immune response; Immunity (Disease); Immunological tolerance; Immunology; Kidneys; Liver; Liver - metabolism; New species; New Zealand; Perciformes - genetics; Perciformes - immunology; Stress, Physiological - genetics; Temperature; Temperature effects; Temperature tolerance
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes15111390

Snapper ( ) is a commercially, recreationally and culturally important teleost species in New Zealand and has been selected as a potential new species for aquaculture. Selective breeding to enhance stress tolerance, survival and growth are major breeding targets, yet research into snapper immune and stress responses has been limited. We explored a set of candidate genes in the fin, head kidney and liver tissues of 50 individuals by exposing 20 fish to increasing temperature (up to 31 °C) and 20 fish to decreasing temperature (down to 7 °C) for up to 37 h. Of these, we analysed 10 temperature-sensitive and 10 temperature-tolerant fish, along with 10 fish kept at 18 °C (acclimation temperature) as a control group. Expression analyses of candidate stress genes in the three tissue types via NanoString Technologies, Inc., Seattle, WA, USA. showed that 20 out of 25 genes significantly changed in each experiment, demonstrating the significant impact of temperature on stress and immune responses. We further document that 10 key gene biomarkers can be used to predict genotypes that are tolerant to extreme temperatures. Taken together, our novel NanoString method can be used to monitor stress in snapper rapidly, and applications of this tool in this and potentially closely related teleost species can provide insights into stress resilience of wild stocks and inform the selection of grow-out locations for aquaculture.