Gene Therapy Approach for Treatment of Obese Agouti Mice

Obesity is a significant metabolic disorder associated with excessive fat accumulation and insulin resistance. In this study, we explored a gene therapy approach to treat obesity in agouti mice using adeno-associated viruses (AAVs) carrying PRDM16, FoxP4, or Follistatin (FST) genes, which are known...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2024-11, Vol.25 (22), p.12144
Main Authors: Yunin, Maxim A, Boychenko, Stanislav S, Lebedev, Petr, Deykin, Alexey V, Pokrovskii, Mikhail V, Egorov, Alexander D
Format: Article
Language:English
Subjects:
Adipocytes
Adipose tissues
Animals
Bioenergetics
Body fat
Body Weight
Brief Report
Care and treatment
Chronic illnesses
Cloning
Complications and side effects
Dependovirus - genetics
Diabetes therapy
Energy metabolism
Energy storage
Follistatin - genetics
Follistatin - metabolism
Forkhead Transcription Factors - genetics
Forkhead Transcription Factors - metabolism
Gene therapy
Genes
Genetic Therapy - methods
Genetic Vectors - genetics
Health aspects
Insulin resistance
Lipids
Male
Mice
Mice, Obese
Obesity
Obesity - genetics
Obesity - metabolism
Obesity - therapy
Patient outcomes
Plasmids
Prevention
Risk factors
Thermogenesis
Transcription Factors - genetics
Transcription Factors - metabolism
Triglycerides - metabolism
Weight control
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Obesity is a significant metabolic disorder associated with excessive fat accumulation and insulin resistance. In this study, we explored a gene therapy approach to treat obesity in agouti mice using adeno-associated viruses (AAVs) carrying PRDM16, FoxP4, or Follistatin (FST) genes, which are known to promote the browning of white adipose tissue. Mice treated with AAVs encoding PRDM16, FoxP4, or FST genes showed a reduction in body weight (10-14%) within the first three weeks after administration, compared to the control groups. A lipidomic analysis of the adipose tissue revealed a dramatic reduction in triacylglycerol (TAG) species with low carbon numbers (40-54 acyl carbons) in treated mice.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252212144